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. 2002 Apr 23;99(9):6181–6186. doi: 10.1073/pnas.092141999

Figure 1.

Figure 1

ATAC, MIP-1α, MIP-1β, RANTES, and IFN-γ are cosecreted in polyclonally activated NK cells, CD8+ T cells, and Th1-differentiated CD4+ T cells. C57BL/6 splenocytes were stimulated with phorbol 12-myristate 13-acetate, ionomycin, and brefeldin A for 6 h, stained for DX5 or CD8, fixed, and counterstained with the respective cytokine/chemokine reagents. (A) Gate on DX5+ cells. (B) Gate on CD8+ cells. The correlation coefficient φ is indicated in the right upper quadrants. Shown is representative of four experiments. (C) OVA-specific Th1 or Th2 cells were restimulated after 2 weeks of differentiation with phorbol 12-myristate 13-acetate, ionomycin, and brefeldin A for 6 h and analyzed for the expression of ATAC, the CC chemokines, IFN-γ, and IL-4. Black profiles indicate isotype controls. The data given were obtained in one representative experiment out of two.