Table 1.
Validation of microarray data by QPCR
| Gene of interest (GOI) | Treatment | GOI average CT | GAPDH average CT | ΔCT GOI − GAPDH | ΔΔCT ΔCT − ΔCTMCS | Fold change relative to MCS |
|---|---|---|---|---|---|---|
| Pitpn (U96726) | FIVMCS | 25.02 ± 0.80 | 19.18 ± 0.18 | 5.84 ± 0.82 | 0.00 ± 0.82 | 1.0 |
| FIVβgluc | 20.07 ± 0.75 | 21.69 ± 0.46 | −0.99 ± 0.89 | −6.83 ± 0.88 | 114.04 | |
| Fe65 (P46933) | FIVMCS | 28.62 ± 0.52 | 19.18 ± 0.18 | 9.44 ± 0.55 | 0.00 ± 0.55 | 1.0 |
| FIVβgluc | 26.99 ± 0.77 | 21.69 ± 0.46 | 5.30 ± 0.90 | −4.14 ± 0.90 | 17.67 | |
| C/EBPδ (X61800) | FIVMCS | 31.71 ± 0.32 | 19.18 ± 0.18 | 12.53 ± 0.37 | 0.00 ± 0.37 | 1.0 |
| FIVβgluc | 29.88 ± 0.87 | 21.69 ± 0.46 | 8.19 ± 0.99 | −4.35 ± 0.99 | 20.30 | |
| Egr2 (X06746) | FIVMCS | 22.35 ± 0.35 | 19.18 ± 0.18 | 3.17 ± 0.39 | 0.00 ± 0.39 | 1.0 |
| FIVβgluc | 21.15 ± 0.51 | 21.69 ± 0.46 | −0.54 ± 0.69 | −3.71 ± 0.69 | 13.12 |
TaqMan chemistry was used to measure gene expression by means of real-time quantitative PCR (QPCR; Materials and Methods). A comparative CT method allowed calculation of relative changes in gene expression of mice treated with FIVβgluc vs. FIVMCS. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a reference for all comparative analyses because microarray data revealed statistical consistency throughout experimental conditions and replicates. t tests on the replicates of each sample achieved P < 0.001 or better for every sample.