Figure 4.

Data representing varying input concentrations. (A) Linearity assessment using the cfDNA reference material spiked into 2 mL of plasma at input concentrations ranging from 10 ng to 200 ng. The extraction demonstrated excellent linearity and high recovery efficiency with a strong correlation (R² ≥ 0.99) using the Revolution workflow. (B) Representative TapeStation electropherogram showing cfDNA fragment size distribution, with distinct peaks at approximately149 bp (mononucleosomal), 358 bp (dinucleosomal), and 563 bp (trinucleosomal).