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. 2025 Jul 11;14(14):1065. doi: 10.3390/cells14141065

Figure 4.

Figure 4

(A,B) NTA quantification of particle concentration in plasma-derived sEVs from naïve and injured mice in the subacute (A) and chronic (B) phases post-SCI. (C,D) NTA comparison of mean particle size for naïve versus injured plasma-derived sEVs in the subacute (C) and chronic (D) phases. One-way ANOVA followed by Tukey’s multiple comparison test was used to assess statistical significance. (E,F) MACSPlex analysis of tetraspanins (CD81, CD63, CD9) on plasma-derived sEVs from naïve and subacutely injured mice (E) and from naïve and chronically injured mice (F). Two-way ANOVA followed by Tukey’s multiple comparisons test was used to assess significance across tetraspanin markers. All experiments were performed in triplicate, with error bars representing the standard error of the mean. Significance is indicated as follows: ** p  < 0.01, *** p  < 0.001, **** p  < 0.0001. All experiments were performed in triplicate; error bars represent the standard error of the mean.