Figure 5.

Electrical field stimulation-induced BDNF secretion depends on Ca²⁺ mobilization from intracellular stores. In the first set of experiments Ca²⁺ stores were depleted by preincubation with caffeine (10 mM) and thapsigargin (10 μM) for 20 min. This pretreatment abolished subsequent BDNF secretion by 50 Hz field stimulation (n = 33). In a second experiment hippocampal neurons were preincubated (2–20 min) with 75 μM 2-APB, an inhibitor of IP₃ receptor function. 2-ABP blocked BDNF secretion induced by 50 Hz field stimulation (n = 42). Values given represent the mean ± SE. n.s., Not significant (P > 0.05); **, P < 0.01.