Figure 1.

Induction of the PI-II gene in response to wounding and MeJA. Two-leaf stage WT and mutant (spr-2 and jai-1²) tomato plants were wounded once with a hemostat across the midvein of the lower leaf. Leaf tissue was harvested separately (8 hr later) from the wounded leaf (L, local response) and the upper undamaged leaf (S, systemic response) for RNA isolation. RNA was also isolated from a set of plants treated for 12 h with MeJA (J) and a set of untreated control plants (C). Aliquots (5 μg) of total RNA were analyzed by RNA blot analysis for PI-II mRNA levels. As a loading control, a duplicate blot was probed with a cDNA encoding eIF4A.