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. 2002 Apr 30;99(9):6422–6427. doi: 10.1073/pnas.092141899

Figure 2.

Figure 2

Yeast two-hybrid assay showing that the AtTOR FRB domain is able to form a complex with rapamycin and ScFKBP12. (A) Two-hybrid strain SMY87–4 coexpressing the GAL4(BD)∷AtFRB and the GAL4(AD)∷ScFKBP12 fusion proteins was spread on medium lacking adenine. Formation of the FKBP12–rapamycin–FRB complex induces expression of the GAL-ADE2 reporter gene and is revealed by growth around the rapamycin (Rap.) disk (Right). (B and C) Same experiment as A, performed with control isogenic strains coexpressing the unfused GAL4(BD) and the GAL4(AD)∷ScFKBP12 fusion protein (B) or the GAL4(BD)∷AtFRB fusion protein and the unfused GAL4(AD) (C).