Fig. 2.

Differentiation protocols for PSCs-derived lung organoids. (A) A 2D differentiation method for generating lung epithelial cells (i) and a protocol illustrating the generation lung bud organoids (ii). Reproduced with permission from Ref [53]., Copyright 2017, Springer Nature. (B) A stepwise differentiation for generating proximal airway organoids. Reproduced with permission from Ref [58]., Copyright 2016, Cell. (C) A protocol for deriving alveolar organoids from iPSCs. Reproduced with permission from Ref [54]., Copyright 2017, Springer Nature. (D) An alveolosphere differentiation protocol from human PSCs. Reproduced with permission from Ref [55]., Copyright 2019, Springer Nature. (E) An airway-directed differentiation protocol from human PSCs. Reproduced with permission from Ref [56]., Copyright 2021, Cell. (F) An airway and alveolar organoids differentiation protocol from iPSC-derived NKX2-1 + lung bud tip progenitor organoids. Reproduced with permission from Ref [57]., Copyright 2022, Biologists