Table 2.

eDNA-deficient M. avium A5 mutants

Location of transposon	Mutant	eDNAa (d7/O.D.)	G-C conent	Conserved domain	Neighboring genesc
MAVA5_03380	11e7	6177	61.6	FtsK/SpoIIIE (DNA pore ATPase)	Hypothetical, plasmid replication, integration, and excision activator. Integrase, tRNA-Phe, tRNA-Asp, tRNA-glu, tRNA-Lys
MAVA5_10275b	7d3	9918	57.8	Metal-dependent hydrolase	TetR family transcriptional regulator. acetyl-CoA carboxylase, acetyl-CoA carboxylase subunit alpha, acyl-CoA dehydrogenase, long-chain fatty acid-CoA ligase, TetR family transcriptional regulator
MAVA5_13430	9e11	19,286	71.8	PknB (Serine/threonine kinase)	Hypothetical, hypothetical, hypothetical, exinulease ABC subunit UvrB/DNA Polymerase III, EmrB efflux pump/Major facilitator superfamily

^aWT A5 eDNA (d7/O.D) was ~ 30,000

^bGene is in 50 kb genomic region that is associated with DNA export

^cItalics: downstream, Normal: upstream