NEUROBIOLOGY. For the article “Identification of genes expressed with temporal-spatial restriction to developing cerebellar neuron precursors by a functional genomic approach,” by Qing Zhao, Alvin Kho, Anna Marie Kenney, Dong-in Yuk, Isaac Kohane, and David H. Rowitch, which appeared in number 8, April 16, 2002, of Proc. Natl. Acad. Sci. USA (99, 5704–5709), column headings in Table 1 did not appear correctly due to a printer's error. The corrected table appears below.
Table 1.
Expression of genes/ESTs in vivo in the neonatal mouse cerebellum
| Genes | Accession no. | PN 1 EGL | PN 7
|
PN 15
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| EGLa | EGLb | PK | IGL | EGLa | EGLb | PK | IGL | |||
| Training set: EGL expression—positive* | ||||||||||
| CycD1 | W08016 | +++ | +++ | − | − | − | − | − | − | − |
| STK1 | D21099 | +++ | ++ | ++ | − | − | − | − | − | − |
| Cdc20 | AA00468 | +++ | +++ | +++ | − | − | + | + | + | + |
| CKS2 | AA11263 | ++ | ++ | − | − | − | − | − | − | − |
| AYK1 | U80932 | +++ | ++ | − | − | − | + | + | − | − |
| IPL1 | AA050055 | + | ++ | ++ | − | − | − | − | − | − |
| SET | W78604 | +++ | +++ | +++ | − | − | + | + | + | + |
| BM28 | AF004105 | +++ | +++ | − | − | − | − | − | − | − |
| RanBP1 | X56045 | +++ | +++ | − | − | − | − | − | − | − |
| DP1 | X72310 | ++ | +++ | ++ | − | − | − | − | − | ++ |
| Training set: EGL expression—negative* | ||||||||||
| Stra13 | Y07836 | − | − | − | − | − | − | − | − | ++ |
| EST69† | C76791 | − | − | +/− | ++ | + | − | − | − | − |
| Test set: EGL expression—positive‡ | ||||||||||
| E2F1 | L21973 | + | ++ | ++ | − | − | − | − | − | ++ |
| RNM2 | M14233 | ++ | +++ | +++ | − | − | − | − | − | − |
| CycB2 | X66032 | +++ | ++ | ++ | − | − | − | − | − | − |
| N-myc | X03919 | +++ | +++ | +++ | − | − | − | − | − | − |
| CycA2 | Z26580 | +++ | +++ | +++ | − | − | − | − | − | − |
| CycB1 | AA426917 | ++ | +++ | +++ | − | − | − | − | − | − |
| mMIS5 | AA689977 | +++ | +++ | +++ | − | − | − | − | − | − |
| EIF4A | AA166088 | ++ | +++ | +++ | + | + | − | − | − | − |
| HMG1 | Z11997 | ++ | +++ | +++ | − | − | − | − | − | − |
| PAL31 | W48027 | ++ | +++ | +++ | + | + | − | − | − | − |
| Test set: EGL expression—negative‡ | ||||||||||
| AA84 | AA688784 | − | + | + | − | − | − | − | − | − |
| AA96 | AA267296 | − | + | + | − | − | − | − | − | − |
| FKBP-13† | AA072278 | − | +/− | +/− | − | − | − | − | − | − |
| BC73 | AA16900 | − | +/− | +/− | + | − | − | − | − | − |
| RCC | AA051583 | − | + | + | − | − | − | − | − | − |
| AA88 | AA275288 | − | + | + | − | − | − | − | − | − |
| QKI5A | AA174970 | − | − | − | +++ | +++ | − | − | − | − |
| FIN-14 | U42386 | − | − | − | ++ | ++ | − | − | − | − |
PK, Purkinje cell; IGL, internal granule layer; EGLa, actively proliferating granule cells; EGLb, granule cells that recently have left the cell cycle.
A training set of 12 genes was tested for EGL-specific expression in PN 1, 7, and 15 mouse cerebella by ISH. Ten of these (CycD1, STK1, CDC20, CKS2, AYK1, IPL1, SET, BM28, RanBP1, and DP1) showed expression in the PN 7 EGL in contrast to Stra13 and EST69.
Entries identified by the TRP-EGL that failed to show expression in the EGL in vivo (false positives). False negatives, not identified by the TRP-EGL but having EGL-specific expression, are underlined.
Test data set expression in vivo. Of an additional 18 genes tested, 10 were expressed in the PN 7 EGL, and 8 genes were not expressed specifically in the EGL. The relative intensity of expression is shown as strong (+++), moderate (++), weak (+) or absent (−).