Skip to main content
. 2025 Jun 21;17(7):876. doi: 10.3390/v17070876

Figure 1.

Figure 1

TRIM5α limited lentivirus infection efficiency in MDBK cells. (A) MDBK and 293T cells were infected with different amounts of HIV-1-based lentivirus encoding a luciferase reporter protein (30, 60, 90 μL). Luciferase expression was determined at 48 h post-infection. (B) MDBK and 293T cells were infected with different amounts of HIV-1-based lentivirus encoding an EGFP reporter protein (30, 60, 90 μL). EGFP (green), a surrogate marker of infection, and nuclei were stained with DAPI (blue) and observed with a fluorescence microscope. (C) RT-PCR and Western blotting were used to measure the mRNA and protein expression of TRIM5α in MDBK cells that were transfected with siRNA targeting bovine TRIM5α or scramble siRNA, and the relative protein expression level of TRIM5α was shown with densitometry. (D) MDBK cells transfected with siRNA targeting TRIM5α or scramble siRNA were infected with different amounts of HIV-1-based lentivirus encoding an EGFP reporter protein (30, 60, 90 μL). The fluorescence, a surrogate marker of infection, was observed with a fluorescence microscope at 48 h post-infection. (E) MDBK cells transfected with siRNA targeting TRIM5α or scramble siRNA were infected with different amounts of the HIV-1-based lentivirus encoding a luciferase reporter protein (30, 60, 90 μL). Luciferase expression was determined at 48 h post-infection. The results are presented as the mean and standard deviation of triplicate measurements from one assay, and they are representative of at least three independent experiments. Differences were examined with a two-tailed, unpaired Student’s t-test. **** p < 0.0001.