Table 3.
Genetic profile based on both cox1 RD-PCR and ITS-2/18S sequence analysis of eggs successfully genotyped (n) by country of origin and type of sample (urine or stool).
| Genotyped eggs | Country | (n) | RD-PCR | Sequence | Mito-nuclear signature | Genetic profile | Classification | ||
|---|---|---|---|---|---|---|---|---|---|
| Sh cox1 | Sb or Sc cox1 | Sm cox1 | ITS-2 | ||||||
| Eggs from urine n = 642 | The Gambia | 15 | 15 | — | — | ShSh | ShxShSh | S. haematobium | Pure |
| Guinea-Bissau | 36 | 35 | — | — | ShSh | ShxShSh | S. haematobium | Pure | |
| 1 | — | — | ShSb/Sc ∗ | ShxShSb/Sc ∗ | S. haematobium × S. bovis/S. curassoni | Hybrid | |||
| Côte d'Ivoire | 12 | 8 | — | — | ShSh | ShxShSh | S. haematobium | Pure | |
| 4 | — | — | ShSc | ShxShSc | S. haematobium × S. curassoni | Hybrid | |||
| Mali | 249 | 195 | — | — | ShSh | ShxShSh | S. haematobium | Pure | |
| — | — | 40 | — | ShSh | SbxShSh | S. haematobium × S. bovis | Hybrid | ||
| — | — | 5 | — | ShSc | SbxShSc | S. haematobium × S. bovis × S. curassoni | Hybrid | ||
| — | 1 | — | — | ShSb/Sc ∗ | ShxShSb/Sc ∗ | S. haematobium × S. bovis/S. curassoni | Hybrid | ||
| — | 5 | — | — | ShSc | ShxShSc | S. haematobium × S. curassoni | Hybrid | ||
| — | 3 | — | — | ShSb | ShxShSb | S. haematobium × S. bovis | Hybrid | ||
| Mauritania | 128 | 109 | — | — | ShSh | ShxShSh | S. haematobium | Pure | |
| — | — | 10 | — | ShSh | SbxShSh | S. haematobium × S. bovis | Hybrid | ||
| — | — | 2 | — | ShSb | SbxShSb | S. haematobium × S. bovis | Hybrid | ||
| — | 7 | — | — | ShSc | ShxShSc | S. haematobium × S. curassoni | Hybrid | ||
| Senegal | 202 | 173 | — | — | ShSh | ShxShSh | S. haematobium | Pure | |
| — | — | 27 | — | ShSh | SbxShSh | S. haematobium × S. bovis | Hybrid | ||
| — | 2 | — | — | ShSc | ShxShSc | S. haematobium × S. curassoni | Hybrid | ||
|
| |||||||||
| Eggs from stool n = 60 | Guinea | 12 | — | — | 12 | SmSm | SmxSmSm | S. mansoni | Pure |
| Equatorial Guinea | 9 | — | — | — | SgSg | SgxSgSg ∗∗ | S. guineensis | Pure | |
| Senegal | 31 | — | — | 31 | SmSm | SmxSmSm | S. mansoni | Pure | |
| Côte d'Ivoire | 8 | — | — | 8 | SmSm | SmxSmSm | S. mansoni | Pure | |
Note: In bold = eggs with double chromatogram peaks at the polymorphic positions that discriminate between S. haematobium and S. bovis/S. curassoni in their ITS-2 sequences; in this case, 18S sequences were used for species identification.
Abbreviations: Sb, S. bovis; Sc, S. curassoni; Sg, S. guineensis; Sh, Schistosoma haematobium; Sm, S. mansoni.
∗ Indeterminate due to the lack of DNA for 18S rRNA gene sequencing.
∗∗ Mito-nuclear signature performed on the information obtained from mtDNA cox1 sequencing.