Figure 4.

In Vitro interaction of PR with SRC-1 or SMRT. (A) Interactions between PR and SRC-1. The full-length SRC-1 synthesized in oocytes was first incubated with anti-FLAG M₂ resin, then incubated with His-tag PR in the presence of vehicle, P, or RU486. The bound proteins were subjected to Western blotting of PR and SRC-1. (B) Interactions between PR and SMRT. Sf9 cells were coinfected with PR and SMRT baculovirus. Whole cell extracts were prepared and incubated with anti-PR antibody conjugated to protein A argarose beads in the presence of vehicle, P, or RU486. The proteins bound to the beads were subjected to the Western blot analysis with antibodies against SMRT or PR.