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. 2025 Jul 14;13(7):1661. doi: 10.3390/microorganisms13071661

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© 2025 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Protection against chronic toxoplasmosis in immunized mice two weeks after the final booster immunization. (A) Cyst reduction in immunized BALB/c mice, n = 6/group. (B) Cyst reduction in immunized C57BL/6 mice, n = 6/group. (C) Cyst reduction in immunized Kunming mice, n = 6/group. Bars represent the mean cyst burden per mouse brain following an oral challenge with 10 cysts of the ME49 strain. Cyst load was determined from whol-brain homogenates collected four weeks post-challenge. Data are presented as means ± SD (representative of three experiments). Statistical significance is indicated as *** p < 0.001, and ** p < 0.01 compared to control groups.