Figure 5.

Competition for CCT substrate binding by native PhLP. Urea-denatured ¹²⁵I-G_tα was diluted 100-fold into rabbit reticulocyte lysate to a final concentration of 1.0 μM. Native PhLP was added to a final concentration of 0.5, 2.0, and 10.0 μM. CCT was immunoprecipitated by using the anti-TCP-1α antibody, and the immunoprecipitates were subjected to SDS/PAGE and phosphorimager analysis or immunoblotting. (Upper) An immunoblot for PhLP. The middle panel is the ¹²⁵I-G_tα phosphorimage of one gel. (Lower) A graphical representation of the data from three separate experiments. Error bars represent the standard deviation between experiments.