Figure 7.
In vivo inhibition of actin folding by PhLP. (A) CHO cells were transiently transfected with PhLP or LacZ and the folding of nascent β-actin was measured by the amount of 35S-labeled actin bound to DNase I beads. Error bars represent the standard deviation between six separate experiments. (B) The amount of PhLP in untransfected CHO cells was determined by quantitative immunoblot analysis. Increasing amounts of total protein from cell extracts as well as purified samples of CCT or PhLP were immunoblotted (IB) with anti-TCP-1α or PhLP antibodies and band intensities between standards and cell extracts were compared with determine the amount of CCT and PhLP in the CHO cells. From these data a ratio of holo-CCT to PhLP of 3.5 ± 0.5 to 1 was calculated.