Secondary Metabolites in Plants

1. Introduction

Classically, plant metabolism has been divided into primary and secondary metabolism, although nowadays there is a broad interface between them that makes this classification increasingly difficult to uphold. In general, primary metabolic pathways are those present in all plant species and throughout most of a plant’s life cycle, as they are essential for survival and determine growth. In contrast, secondary metabolic pathways are not universal; the biosynthesis and accumulation of secondary metabolites (SMs) are associated with specific developmental stages, linked to the specialization of certain organs and tissues, or to particular moments in the plant’s life that are highly dependent on environmental conditions (Figure 1).

Figure 1.

Role of primary and secondary metabolism in plant development.

Thus, it can be stated that plant secondary metabolism is a source of a plethora of chemical signals that shape plant interactions with their environment, playing key roles in processes such as pollination, seed dispersal mediated by herbivores, and defense against pathogens and other predators, among others [1] (Figure 2).

Figure 2.

Ecological roles of the plant secondary metabolites.

This tenuous and artificial boundary between primary and secondary metabolism implies that some vital and ubiquitous compounds in higher plants—such as phytosterols and hormones like gibberellins and abscisic acid—are produced through secondary metabolic pathways, such as the terpenoid biosynthetic route, despite their essential roles across plant taxa [2,3]. In fact, hormonal regulation, including that of abscisic acid (ABA) and salicylic acid (SA), has been shown to influence the flux of terpenoid biosynthesis under abiotic stress conditions, further blurring the line between primary and secondary metabolism [4].

Primary metabolism supplies plants with small molecules such as simple sugars, amino acids, and fatty acids—compounds ubiquitous across the plant kingdom—which, through biochemical conversions, give rise to macromolecules like polysaccharides, proteins, nucleic acids, and lipids, which define plant structure and support growth. From these major metabolic branches—nitrogen, lipid, and carbohydrate—an immense diversity of secondary compounds emerges through specific metabolic pathways, including phenolics, terpenoids, and alkaloids, among others (Figure 3). Most of these biosynthetic pathways remain largely uncharacterized, yet their study is of vital importance, as many of the resulting compounds have strong biological activities and are widely used in the pharmaceutical, cosmetic, and food industries [5].

Figure 3.

Biogenetic classification of plant secondary metabolites.

The growing market demand for natural plant products has threatened numerous plant species in their natural habitats and prompted the development of new strategies for sustainable production. In this context, plant biotechnology and, in particular, the development of so-called plant biofactories and the application of metabolic engineering techniques offers a more eco-sustainable alternative for producing these valuable compounds [6]. On the other hand, plant biofactories can also serve as powerful tools for elucidating secondary biosynthetic pathways, thereby expanding the range of possibilities offered by metabolic engineering to enhance the production of secondary metabolites. In this context, recent discoveries have identified novel enzymes involved in the biosynthesis of the important anticancer compound, paclitaxel (Taxol) [7].

These advances are further supported by synthetic biology efforts to reconstruct entire biosynthetic routes in heterologous systems, revealing the minimal gene sets required for paclitaxel biosynthesis and paving the way for controlled, large-scale production [8].

2. Special Issue Overview

This Special Issue, “Secondary Metabolites in Plants”, aims to reveal the functions and biosynthesis of SMs in plants, the biotechnological production of SMs, their biological activities, and the phytochemical characterization of plants, in search of new bioactive compounds. The Special Issue compiles fifteen contributions (eleven original articles and four reviews) that address several aspects of alkaloids, terpenes, and phenolic and volatile compounds.

2.1. Alkaloid Metabolism

The alkaloid accumulation in capsules of two industrial opium poppy (Papaver somniferum) varieties was analyzed using different capsule sizes and tissues. It revealed genotype-specific patterns of morphinane distribution. The findings highlight how structural and genetic factors influence secondary metabolite profiles. This contributes valuable knowledge to targeted breeding for enhanced alkaloid production [9].

2.2. Phenol Metabolism

The elicitors and genetic mutations enhance phenolic biosynthesis, with Petrova et al. [10] demonstrating yeast extract’s role in boosting caffeoylquinic acid in Arnica montana, while Li et al. [11] associated elevated phenolics with antioxidant activity and leaf color in a Lagerstroemia mutant.

Advances in enzyme evolution and regulatory networks are reshaping our understanding of phenolic biosynthesis, with Zhou et al. [12] tracing the origin of Mentha longifolia acyltransferases to gene duplication events, while Tan et al. [13] reveal how kinase-transcription factor interactions govern secondary metabolism in Forsythia.

Innovative biotechnological approaches are catalyzing the sustainable production of valuable plant metabolites, with Suprun et al. [14] identifying Reynoutria japonica as a superior stilbene source and developing optimized culture systems, while Sharma et al. [15] have demonstrated the potential of Dalea purpurea hairy root cultures to efficiently produce bioactive flavonoids with therapeutic applications.

Cutting-edge research is uncovering novel sources and applications of bioactive plant compounds, with Morante et al. [16] demonstrating the therapeutic promise of Moraceae prenylated flavonoids for drug development, while Khamsaw et al. [17] have revealed banana peel’s untapped potential as a sustainable source of health-promoting phenolics and prebiotics for functional foods.

2.3. Terpene Metabolism

Emerging biotechnological and ecological insights are revolutionizing terpenoid research, with Alcalde et al. [18] engineering Centella asiatica hairy roots for enhanced centelloside production, Cheng et al. [19] synthesizing critical knowledge on terpenoid biosynthesis pathways, and Shakeel et al. [20] advocating for saponin-based solutions in sustainable agriculture.

2.4. Volatile Compounds

Environmental factors and biotic interactions significantly shape plant volatile profiles, as demonstrated by Nurcholis et al. [21], who showed how shading enhances the essential oil bioactivity of Curcuma xanthorrhiza, Sun et al. [22] revealing virus-induced changes in lemon terpenes that alter insect attraction, and Charoimek et al. [23] highlighting abiotic stress effects on Rosa damascena fragrance compounds.

3. Concluding Remarks

The manuscripts featured in this Special Issue explore the fascinating diversity of plant secondary metabolism, offering cutting-edge insights into biosynthesis, regulation, and ecological and biotechnological applications. As Guest Editors of this Special Issue, we are grateful to the authors for their valuable contributions, which will advance our understanding of how plants produce and utilize these specialized compounds. We also extend our sincere appreciation to the reviewers for their constructive feedback and to the Editorial Office for their unwavering support in bringing this collection to fruition.

Conflicts of Interest

The authors declare no conflict of interest.