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. 2025 Jul 14;18:1619583. doi: 10.3389/fnmol.2025.1619583

Figure 2.

Bar charts depicting the mRNA (A) and protein (B) levels of Brd2, Brd3, and Brd4. In chart A, Brd4 shows a significant increase in mRNA levels in MIA compared to the control, indicated by three asterisks. Charts use control and MIA groups, with results presented in relative quantification and arbitrary units.

The effect of MIA on the expression of BET proteins in the hippocampus. PIC (20 mg/kg b.w.) was injected intraperitoneally at gestation day 17 to female mice. Twelve-month-old offspring male mice were decapitated, and the brain tissue was collected. (A) The levels of mRNA for Brd2, Brd3, and Brd4 genes in the hippocampus were measured by using qPCR and calculated by the ΔΔCt method. An averaged Ct-value from three various reference genes (Gusb, Hprt, Rn18s) was used for calculation. (B) The levels of Brd2, Brd3, and Brd4 proteins in the hippocampus were determined by using ELISA assays and normalized to the total protein level. The presented data are means ± SEM. *** p < 0.001, compared with the control group. N = 7–8 (A) and 6 (B). Each data point (○) represents an individual animal. Statistical analyses were conducted using two-way ANOVA followed by the Bonferroni post hoc test, selected based on data distribution.