Figure 4.

The effect of MIA and OTX-015 on Tau level and phosphorylation. PIC (20 mg/kg b.w.) was injected intraperitoneally at gestation day 17 to female mice. An inhibitor of BET proteins, OTX-015 (100 mg/kg b.w. daily), was administered orally to 12-month-old offspring males for 14 days (weeks 50–51), then animals were decapitated, and the brain tissue was collected. (A–E) The level and phosphorylation of Tau protein were analyzed in the hippocampus using Western blotting. Data were normalized on the immunoreactivity of Gapdh. The presented data are means ± SEM. N = 6–8 (A,B), 7–8 (C), 6–7 (D) and 5–7 (E). Each data point (○) represents an individual animal. (F) Three representative sets of Western blot images are shown, derived from 12 individual animals. Statistical analyses were conducted using one-way ANOVA followed by the Bonferroni post hoc test, selected based on data distribution.