Figure 5.

The effect of MIA and OTX-015 on microglial morphology and density in the hippocampus. PIC (20 mg/kg b.w.) was injected intraperitoneally at gestation day 17 to female mice. An inhibitor of BET proteins, OTX-015 (100 mg/kg b.w. daily), was administered orally to 12-month-old offspring males for 14 days (weeks 50–51), then animals were perfused and brain tissue slices were subjected to immunohistochemical analysis using anti-Iba1 antibodies. (A) Representative pictures illustrating Iba1-specific cells (microglia) in the CA1 region of the hippocampus were presented. Scale bar = 20 μm. (B) Semi-quantitative analysis of the number of visible Iba1-positive microglial cells per 1 mm² in the CA1, CA2/3, and DG regions of the hippocampus. (C) The level of a marker of microglia (Iba1) was analyzed by Western blotting. Data were normalized on the immunoreactivity of Gapdh. (D) Three representative sets of Western blot images are shown, derived from 12 individual animals. N = 3 (B) and 7–8 (C). Each data point (○) represents an individual animal. The counting results from the individual images that contribute to each animal’s score are shown as colored diamonds (◊), with each color representing a different animal. Statistical analyses were conducted using one-way ANOVA followed by the Bonferroni post hoc test, selected based on data distribution.