|
| Antibodies |
|
| PE-anti-human T-bet (QA18A24) |
Biolegend |
Cat# 365904; RRID: AB_3675491 |
| Pacific Blue-mouse IgG1κ, isotype ctrl. (MOPC-21) |
Biolegend |
Cat#400151; RRID: AB_3675489 |
| Pacific Blue-anti-human/mouse T-bet (4B10) |
Biolegend |
Cat# 644808; RRID: AB_1595479 |
| FITC-mouse IgG1κ, isotype ctrl. (MOPC-21) |
Biolegend |
Cat# 400138; RRID: AB_3675490) |
| FITC-anti-human CD3 (HIT3a) |
Biolegend |
Cat# 300306; RRID: AB_314041) |
| Brilliant Violet 421-anti-mouse CD4 (GK1.5) |
Biolegend |
Cat# 100437; RRID: AB_10900241 |
| Brilliant Violet 421-anti-human FOXP3 (206D) |
Biolegend |
Cat# 320124; RRID: AB_2565972 |
| Alexa Fluor 647-anti-mouse IL-10 (JES5-16E3) |
Biolegend |
Cat# 505014; RRID: AB_493511 |
| PE-anti-mouse CD11a (I21/7) |
Biolegend |
Cat# 153104; RRID: AB_2716034 |
| Alexa Fluor-647 anti-human IL-10 (JES3-9D7) |
Biolegend |
Cat# 501412, RRID: AB_493318 |
| Brilliant Violet 711-anti-human IFN-γ(4S.B3) |
Biolegend |
Cat# 502540; RRID: AB_2563506 |
| PE-anti-mouse CD25 (A18246A) |
Biolegend |
Cat# 113703; RRID: AB_2927943 |
| APC-anti-mouse CD25 (PC61) |
Biolegend |
Cat# 102012; RRID: AB_312861 |
| FITC-anti-mouse CD45RB (C363-16A) |
Biolegend |
Cat# 103305: RRID: AB_313012 |
| PE-Cyanine7-anti-mouse CD49d (R1-2) |
Biolegend |
Cat# 103618; RRID: AB_2563700 |
| PerCp/Cyanine5.5-anti-mouse CD45.1 (A20) |
Biolegend |
Cat# 110728; RRID: AB_893346 |
| APC-Cyanine7-anti-mouse CD45.1 (A20) |
Biolegend |
Cat# 110716; RRID: AB_313505 |
| APC-anti-human CD56 (5.1H11) |
Biolegend |
Cat# 362504; RRID: AB_2563912 |
| FITC-anti-mouse CD45.2 (104) |
Biolegend |
Cat# 109806; RRID: AB_313442 |
| Brilliant Violet 510-anti-mouse CD90.2 (Thy1.2) (53-2.1) |
Biolegend |
Cat# 140319; RRID: AB_2561395 |
| Alexa Fluor 488-anti-mouse CD107a (1D4B) |
Biolegend |
Cat# 121608; RRID: AB_571983 |
| Alexa Fluor 488-anti-mouse CD107b (M3/84) |
Biolegend |
Cat# 108510; RRID: AB_493308 |
| Brilliant Violet 605-anti-mouse CD4 (GK1.5) |
Biolegend |
Cat# 100451; RRID: AB_2564591 |
| PE-anti-mouse FOXP3 (MF-14) |
Biolegend |
Cat# 126404; RRID: AB_1098117 |
| Rabbit anti-human HSP90 (C45G5) |
Cell Signaling Technology |
Cat# 4877; RRID: AB_2233307 |
| Rabbit anti-human PTGIS |
LS bio |
Cat# LS-C372587; RRID: AB_3675478 |
| Rabbit anti-human PTGIR |
LS bio |
Cat# LS-B11456-50; RRID: AB_3675477 |
| PE-anti-mouse IL-10 (JESS-16E3) |
Invitrogen |
Cat# 12-710182; RRID: AB_466176 |
| APC-anti-mouse IL-17A (TC11-1810.1) |
Biolegend |
Cat# 11-7311-82; RRID: AB_465412 |
| Rabbit anti-human β-Actin |
LS bio |
Cat# LS-B1040-50; RRID: AB_968470 |
| Alexa Fluor 488-anti-mouse IFN-γ (XMG1.2) |
Biolegend |
Cat# 505813; RRID: AB_493312 |
| PE-anti-mouse IL-17A (TC11-18H10.1) |
Biolegend |
Cat# 506904; RRID: AB_315464 |
| Anti-mouse IL-1R2 (AF563) |
R & D Systems |
Cat# AF563; RRID: AB_2125055 |
| Brilliant Violet 711-anti-mouse/human Ki67 (B56) |
BD Biosciences |
Cat# 563755; RRID: AB_2738406 |
| PE-anti-human IL-1RII Antibody (34141) |
R & D Systems |
Cat# FAB663P; RRID: AB_1964613 |
| Brilliant Violet 510-anti-mouse TCRβ (H57-597) |
Biolegend |
Cat# 109234; RRID: AB_2562350 |
| PE-anti-mouse IL-17A (TC11-18H10.1) |
Biolegend |
Cat# 506904; RRID: AB_315464 |
| Goat anti-human IL-1RII Antibody |
R & D Systems |
Cat# AF-263-NA; RRID: AB_354431 |
| Rabbit anti-human PTGER4 |
Thermo Fisher Scientific |
Cat# PA5-18476; RRID: AB_10981059 |
| Anti-human COX-2 (495222) |
R & D Systems |
Cat # 612596; RRID: AB_399879 |
| APC-anti-goat IgG |
R & D Systems |
Cat# F0108; RRID: AB_573124 |
| Mouse monoclonal IgG (20116) |
R & D Systems |
Cat# MAB004; RRID: AB_357346 |
| APC-anti-human IL-10 |
Biolegend |
Cat# 506807; RRID: AB_315457 |
| PE-Violet 770-anti-human IFN-γ (45-15) |
Miltenyi Biotec |
Cat# 130-113-499; RRID: AB_2726168 |
| APC-anti-human CD4 (OKT4) |
Biolegend |
Cat# 317416; RRID: AB_571945 |
| APC-anti-human CD69 (FN50) |
Biolegend |
Cat# 310910; RRID: AB_314845 |
| APC-anti-human C5aR2 (1D9-M12) |
Biolegend |
342406; RRID: AB_2564331 |
| Alexa Fluor 488-anti-human RORγt (Q21-559) |
BD Biosciences |
Cat# 563621; RRID: AB_2738325 |
| Pacific Blue-anti-mouse IFN-γ (XMG1.2) |
Biolegend |
Cat# 505818; RRID: AB_528922 |
| Alexa Fluor 647-anti-mouse IL-10 (JES5-16E3) |
Biolegend |
Cat# 505818; RIDD: AB_2125096 |
| PE-Cyanine7-anti-human IL-17A (eBio64DEC17) |
Thermo Fisher Scientific |
Cat# 25-7179-42; RRID: AB_11063994 |
| Anti-human PTGER2 (ARC1393) |
Thermo Fisher Scientific |
Cat# MA5-35750; RRID: AB_2849650 |
| Alexa Fluor 488-anti-human IL-17A |
Biolegend |
Cat# 512308; RRID: AB_961386 |
| Anti-mouse CD3 (145-2C11) |
BioXcell |
Cat# BE0001-1; RRID: AB_1107634 |
| Anti-mouse CD28 (37.51) |
BioXcell |
Cat # BE0015-1; RRID: AB_1107624 |
| Anti-human CD3 (OKT3) |
BioXcell |
Cat# BE0001-2; RRID: AB_1107632 |
| Anti-human CD28 (CD28.2) |
BD Biosciences |
Cat # 555726; RRID: AB_396069 |
| Anti-human CD46 (TRA2-10) |
Wash-U Hybridoma Center (WUHC) |
Clone TRA-2-10; RRID: AB_10895912 |
| Rabbit anti-human PTGES2 |
Thermo Fisher Scientific |
Cat# PA5-22213; RRID: AB_11154153 |
| PE-Cy7-anti-Mouse RORγt (Q31-378) |
BD Biosciences |
Cat# 567305; RRID: AB_2916546 |
| TruStain FcX PLUS anti-mouse CD16/32 (S17011E) |
Biolegend |
Cat# 156604; RRID: AB_2783137 |
|
| Bacterial and virus strains |
|
| Influenza PR8-33 (H1N1) |
Mueller et al.84
|
N/A |
|
| Biological samples |
|
| Healthy donor blood buffy coats |
NIH blood Bank |
N/A |
| Blood samples from individuals with CAPS |
NIH, IRB#17-I-0016, Table S3
|
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| Brilliant Violet-conjugated NP311-325 |
NIH Tetramer Core |
N/A |
| tetramer |
|
|
| Human IL-1R2 protein, recombinant |
R & D Systems |
Cat# 263-2R-050/CF |
| C5aR2 agonist (P32, Ac-RHYPYWR-OH) |
Li et al.68
|
N/A |
| Human IL-2 protein, recombinant |
Peprotech |
Cat# 200-02 |
| PGI2 (prostacyclin) |
Cayman Chemicals |
Cat# 14010 |
| PGI2-R agonist (epoprostenol) |
TOCRIS |
Cat# 2989 |
| PGI2-R antagonist |
TOCRIS |
Cat# 4268 |
| PGE2 (prostaglandin E2) |
Cayman Chemicals |
Cat# 14010 |
| EP4 receptor antagonist (L-161,982) |
Sigma |
Cat# SML0690 |
| EP2 antagonist (C52) |
Sigma |
Cat# SML3643 |
| LIVE/DEAD™ Fixable Near-IR |
Invitrogen |
Cat# L10119
|
| LIVE/DEAD™ Fixable Aqua |
Invitrogen |
Cat# L34965
|
| Cell trace violet |
Invitrogen |
Cat# C34557
|
| COX-2 inhibitor |
Cayman Chemicals |
Cat# 70210 |
| mPGES-1 inhibitor (MF63) |
Cayman Chemicals |
Cat# 13217 |
| 15-hydroxy PGDH inhibitor |
Cayman Chemicals |
Cat# 18040 |
| TBXAS1 inhibitor |
Cayman Chemicals |
Cat# 70510 |
| PPARγ antagonist |
Cayman Chemicals |
Cat# 10026 |
| Cell Stimulation Cocktail (plus protein transport inhibitors) (500x) |
Thermo Fisher Scientific |
Cat# 00-4975-93 |
| Barbadin |
MedChem Express |
Cat# HY-119706 |
| RIPA Lysis and Extraction Buffer |
Thermo Fisher Scientific |
Cat# 89900 |
| Halt™ Protease Inhibitor Cocktail (100x) |
Thermo Fisher Scientific |
Cat# 78430 |
| Halt Phosphatase Inhibitor Cocktail |
Thermo Fisher Scientific |
Cat# 78420 |
| PKA (protein kinase A) inhibitor KT 5720 |
R & D Systems |
Cat# 1288 |
| 4x Laemmli Sample Buffer |
BioRad |
Cat# 1610747 |
| Trans-Blot Turbo Midi Nitrocellulose Transfer Packs |
BioRad |
Cat# 1704159EDU |
| eBioscience™ Protein Transport Inhibitor Cocktail (500x) |
Thermo Fisher Scientific |
Cat# 00498003 |
| Mouse GM-CSF (granulocyte-macrophage colony-stimulating factor), recombinant |
Biolegend |
Cat# 576304 |
| 1x PBS (phospahe-buffered saline) with 1% Casein |
BioRad |
Cat# 1610783 |
| 2-Mercaptoethanol |
Thermo Fisher Scientific |
Cat# 21985-023 |
| GolgiStop |
BD Biosciences |
Cat# 554724 |
| BD GolgiPlug™
|
BD Biosciences |
Cat# 555029 |
| Collagenase |
Sigma-Aldrich |
Cat# C7657 |
| DNAse I |
Sigma-Aldrich |
Cat# DN25 |
|
| Critical commercial assays |
|
|
Accell Human PTGES2 siRNA
|
HORIZON |
Cat# E-021342-00-0005 |
|
Accell Human PTGIS siRNA
|
HORIZON |
Cat# E-004691-00-0005 |
|
Accell Human PTGIR siRNA
|
HORIZON |
Cat# E-005716-00-0005 |
| Accell Non-targeting Control Pool |
HORIZON |
Cat# D-001910-10-05 |
| Human TaqMan probe RPL7
|
Thermo Fisher Scientific |
CAT# Hs02596927_g1 |
| Human TaqMan probe PTGES2
|
Thermo Fisher Scientific |
CAT# Hs00228159_m1 |
| Human TaqMan probe PTGIR
|
Thermo Fisher Scientific |
CAT# #Hs01125026_m1 |
| Human TaqMan probe PTGIS
|
Thermo Fisher Scientific |
CAT# Hs00919949_m1 |
| Human TaqMan probe TBXAS1
|
Thermo Fisher Scientific |
CAT# Hs01022706_m1 |
| Human TaqMan probe IL1R2
|
Thermo Fisher Scientific |
CAT# Hs00174759_m1 |
| Human TaqMan probe IL10
|
Thermo Fisher Scientific |
CAT# Hs00961622_m1 |
| Mouse TaqMan probe Hprt
|
Thermo Fisher Scientific |
CAT# Mm01545399_m1 |
| Mouse TaqMan probe Ptges2
|
Thermo Fisher Scientific |
CAT# Mm00460181_m1 |
| Mouse TaqMan probe Ptgir
|
Thermo Fisher Scientific |
CAT# Mm00801939_m1 |
| Mouse TaqMan probe Ptgis
|
Thermo Fisher Scientific |
CAT# Mm00447271_m1 |
| PKA Assay Kit |
Abcam |
Cat# 139435 |
| Ebioscience Foxp3/Transcription Factor Staining Buffer Set |
Invitrogen |
Cat# 00-5521-00 |
| Cytofix/Cytoperm Fixation/Permeabilization Solution Kit |
BD Biosciences |
Cat# BDB554714 |
| Mouse CD4 T cell isolation kit |
Miltenyi Biotech |
Cat# 130-104-454 |
| Mouse CD4 T cell isolation kit |
Stem Cell Technologies |
Cat# 19852 |
| Human CD4 T cell isolation kit |
Miltenyi Biotech |
Cat# 130-091-155 |
| Human CD4 T cell isolation kit |
Stem Cell Technologies |
Cat# 17952 |
| EasySep Human CD4+ CD127low CD25+ Regulatory T Cell Isolation Kit |
Stem Cell Technologies |
Cat# 18063 |
| IFN-γ Secretion Assay |
Miltenyi Biotec |
Cat# 130-054-202 |
| IL-10 Secretion Assay |
Miltenyi Biotec |
Cat# 130-090-761 |
| LEGENDplex Human T helper cytokine panel |
Biolegend |
Cat# 741028 |
| LEGENDplex Human Inflammation panel |
Biolegend |
Cat# 740809 |
| LEGENDplex Mouse Inflammation panel |
Biolegend |
Cat# 740446 |
| Human IL-1β/IL-1F2 DuoSet Kit |
R & D Systems |
Cat# DY201 |
| RNAqueous Micro Kit |
Invitrogen |
Cat# AM1931 |
| NEBNext Ultra II RNA Library Prep Kit for Illumina |
NEB |
Cat# E7770 |
| TURBO DNA-free Kit |
Invitrogen |
Cat# AM1907 |
| High-Capacity cDNA Reverse Transcription Kit |
Invitrogen |
Cat# 4387406 |
| TaqMan Universal Master Mix II, no UNG |
Invitrogen |
Cat# 4440043 |
| NEBNext Poly(A) mRNA Magnetic Isolation Module |
NEB |
Cat# E7490 |
|
| Deposited data |
|
| RNA-seq of in vitro activated human CD4+ T cells |
This paper, Figure 3
|
GEO: GSE288921
|
| RNA-seq of in vitro activated mouse CD4+ T cells |
This paper, Figure 6
|
GEO: GSE288969
|
|
| Experimental models: Organisms/strains |
|
| T cells, bone marrow (BM) cells isolated from C57BL/6 littermate control mice to KO lines used |
The Jackson Laboratory |
Strain# 000664 |
| T cells, BM cells isolated from Ptgis-/- mice |
Ge et al.26
|
N/A |
| T cells isolated from Ptgir-/- mice |
NIH, Transgenic Core |
N/A |
| T cells, BM cells isolated from Il1r2-/- mice |
Pinteaux et al.85
|
N/A |
| Mouse strain B6 CD45.1 |
The Jackson Laboratory |
IMSR_JAX:002014 |
| Mouse strain C57BL/10 Rag2-/-
|
Taconic |
Line# 103 |
|
| Software and algorithms |
|
| R (v4.2.2) |
Carvalho et al.86
|
N/A |
| FlowJo 10 software |
Treestar |
https://www.flowjo.com/
|
| FastQC (v0.11.9) |
Andrews87
|
N/A |
| MultiQC (v1.9) |
Ewels et al.88
|
N/A |
| edgeR (v3.40.2) |
Robinson et al.89
|
https://bioconductor.org
|
| GraphPad PRISM 9.4.1 |
Graphpad software |
https://www.graphpad.com
|
| Limma |
Ritchie et al.90
|
https://bioconductor.org
|
| STAR (v2.7.10b) |
Liao et al.91
|
N/A |
| Subread v2.0.3) |
Liao et al.91
|
N/A |
| ggplot2 (v3.4) |
Wickham92
|
N/A |
| ComplexHeatmap (v2.14) |
Gu et al.93
|
N/A |
| Harmony (v1.1) |
Korsunsky et al.94
|
N/A |
| SCPA (v1.5.4) |
Bibby et al.40
|
N/A |
|
| Others |
|
| PBS |
Corning |
Cat# 21-040-CV |
| RPMI |
Thermo Fisher Scientific |
Cat# 21870-076 |
| IMDM |
Thermo Fisher Scientific |
Cat# 12440-053 |
| Lymphoprep separation medium |
Corning |
Cat# 25-072-CI |
| HBSS |
Thermo Fisher Scientific |
Cat# 14170-112 |
| Percoll Cytvia |
Sigma-Aldrich |
Cat# GE17-0891-01 |
| FBS |
Sigma-Aldrich |
Cat# F0926 |
| HEPES |
Corning |
Cat# 25-060-CI |
| Penicillin-Streptomycin |
Thermo Fisher Scientific |
Cat# 15070-63 |
| Glutamine |
Thermo Fisher Scientific |
Cat# 25030-149 |
