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. Author manuscript; available in PMC: 2025 Jul 28.
Published in final edited form as: Cell Rep. 2025 Jun 12;44(6):115858. doi: 10.1016/j.celrep.2025.115858

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER

Antibodies

Anti-Pvalb Swant PV-25
Anti-Pax6 Abcam ab2237
Anti-HuC/D Invitrogen A-21271
Anti-NeuN Millipore MAB377
Goat anti-Mouse Alexa Fluor 647 Abcam ab150115

Experimental Models

Foxd1-Cre C67BL6/CD1 JAX#012463
Isl1-flox C67BL6/CD1 Pan et al.90
Lhx1-flox C67BL6/CD1 -
Nkx2.2-Cre C67BL6/CD1 JAX#026880
Dlx1/2-flox C67BL6/CD1 -
CD1 Wild type mice CD1 Charles River Laboratories

Chemicals, peptides, and recombinant proteins

Hibernate-E Thermo Fisher Scientific Cat# A1247601
Hibernate-A Thermo Fisher Scientific Cat# A1247501
B-27 Supplement Thermo Fisher Scientific Cat# 17504044
GlutaMAX Supplement Thermo Fisher Scientific Cat# 35050061
Hibernate-E Minus Calcium BrainBits Cat# HECA500
Hibernate-A Minus Calcium BrainBits Cat# HACA500
RNase inhibitor Promega Cat# N2615
DNase I Sigma-Aldrich Cat# 4716728001
Trypan Blue Thermo Fisher Scientific Cat# 15250061
ProLong Gold Antifade Mountant Thermo Fisher Scientific Cat# P36934
Aqua-Poly/Mount Fisher Scientific Cat# 18606-20

Critical commercial assays

Chromium Single Cell 3' Library and Gel Bead Kit v3 10x Genomics Cat# 1000075
Chromium Single Cell 3' Library and Gel Bead Kit v3.1 10x Genomics Cat# 1000268
10x Genomics Chromium Single Cell ATAC v1.1 10x Genomics Cat# 1000175
10x Genomics Xenium 10x Genomics Cat# 1000462 + Custom Genes

Deposited data

ScRNA-Seq and scATAC-Seq Raw This paper GSE28449
ScRNA-Seq and scATAC-Seq Processed This paper 10.5281/zenodo.14292830
Xenium Processed This paper 10.5281/zenodo.14292830

Oligonucleotides

Mm-Sp8-C3 ACDBio #547521-C3
Mm-Sp9-C1 ACDBio #564531
Mm-Pitx2-C2 ACDBio #412841-C2
Mm-Irx5-C1 ACDBio #513871
Mm-Meis2-C2 ACDBio #512841-C2
Mm-Npw-C1 ACDBio #495671
Mm-Pnoc-C2 ACDBio #437881-C2
Mm-Slc32a1-C2 ACDBio #319191-C2
Mm-Slc17a6-C3 ACDBio #319171-C3
Mm-Gal-C1 ACDBio #400961

Software and algorithms

CellRanger v5 10x Genomics http://10xgenomics.com
Cell Ranger ATAC (v2) 10x Genomics http://10xgenomics.com
Cytoscape (v3.10.3) https://cytoscape.org/
DeepLabCut (v2.3.4) https://github.com/DeepLabCut/DeepLabCut
All R and Python codes https://github.com/dkim195/HyDD2