Figure 2.

Effects of CCCP and DNP on FtsH-dependent protein degradation in vivo. Cells of AD1377 (ftsH⁺ Δunc; circles) and AD1400 (ΔftsH Δunc; triangles), carrying pSTD466 (yccA; A and B), pSTD181 (atpB; C), or no plasmid (D), were grown in M9 medium at 28°C, pulse-labeled with [³⁵S]methionine from 2 min, and chased in the presence (open symbols) or absence (closed symbols) of either 30 μM CCCP (A, C, and D) or 5 mM DNP (B). For expression of YccA-His₆-Myc and Foa, cells were induced for 10 min with 0.4% arabinose, and 1 mM IPTG plus 5 mM cAMP, respectively, before pulse-labeling. Portions of the samples were withdrawn at the indicated time points and subjected to immunoprecipitation either with anti-Myc, anti-Foa, or anti-σ³².