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. 2025 Jul 8;22(7):812–831. doi: 10.20892/j.issn.2095-3941.2024.0297

Figure 1.

Figure 1

Figure 1

Par3 promotes the metastatic behavior of RCC cells. A. Western blot analysis of Par3 in Caki-1 and ACHN cells. B. Western blot expression of metastatic markers in Par3 overexpressing Caki-1 cell lines (Caki-1vec, Caki-1Par3-2, and Caki-1Par3-4 cells). C. Migration and invasion assays in the Par3 overexpressing Caki-1 cell line. Cells (Caki-1vec, Caki-1Par3-2, and Caki-1Par3-4) were plated in the upper chamber in Transwell plates (8 μm pore size) and assessed at 12 h for migration and at 24 h for invasion experiments. Quantification of three independently performed experiments was performed. D, E. Western blot analysis of (D) metastatic markers and (E) Par3 in ACHN sublines (wt and metastatic sublines). F. qPCR analyses showing transcriptional levels of PARD3 and metastatic gene markers in Par3 knockdown and sc control ACHN brain, ACHN bone, ACHN lung, and ACHN kidney sublines. G. Metastatic ACHN cell line migration and invasion assays. ACHN wt, ACHN bone, and ACHN lung cells were plated in the upper chamber of Transwell plates (8 μm pore size) and assessed at 12 h for migration and at 24 h for invasion experiments. Quantification of three independent experiments was performed. *P < 0.05, **P < 0.01, ***P < 0.001.