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. 2024 Oct 15;74:99–120. doi: 10.1016/j.jare.2024.10.013

Table 2.

Detection of commonly used antimicrobials and bioactive chemicals in agroecosystems.

Contaminant type Residue Sample Detection methods Limit of detection References
Antimicrobials Tetracycline, oxytetracycline and penicillin-G Livestock LC/MS/MS, and LC-ESI-QqQ 1.36, 1.66, and 2.89 µg/kg [142]
Tetracyclines, macrolides, and sulfonamides Soil HPLC-MS/MS 0.01 − 2.00 μg/kg [143]
Fluoroquinolone, quinolone, lincosamide, macrolide, nitroimidazole, pyrimidine, sulfonamide and tetracycline Soil and poultry manure LC-MS/MS 3 μg/kg [51]
Flumequine, ciprofloxacin, enrofloxacin, lincomycin, doxycycline, and oxytetracycline Calf slurry and calf farmyard manure UHPLC-MS/MS 40 and 210 µg/kg [53]
Nitroimidazoles, lincosamides, quinolones, sulfonamides, inhibitors of dihydrofolate reductase, fluoroquinolones, macrolides, tetracyclines, pleuromutilin, antiparasitic, and anti-inflammatory drugs Natural springs UHPLC-QqLIT-MS 0.31 − 3.6 ng/L [144]
Sulfonamides, quinolones, tetracyclines, macrolides, thiamphenicol, and lincomycin Soil from vegetable fields HPLC-MS/MS 0.1 − 20.0 μg/kg [145]
Sulfamethoxazole Soil amended with cattle manure HPLC-UV, LC-MS/MS, and ESI 0.3 and 0.4  μg/kg [146]
Bioactive chemicals Tricyclazole (fungicide), butachlor (herbicide), and clothianidin (insecticide) Rice fields GC-ECD andHPLC 0.005 mg/L [147]
Phytochemicals Leaves MALDI-HRMS Not available [148]
Pesticides Soil HPLC-MS/MS 0.01 − 2.0 μg/kg [143]
Hormones Cattle and pig slurry ESI-LC-MS/MS 1.3 − 6.3 ng/L [55]
Pesticides Wheat farm LC-MS/MS 0.037 and 36 μg/kg [149]
Hormones Crop fields SPE-LC-MS/MS Not available [57]
Pesticides Vegetables LC-MS/MS and GC–MS/MS 1.0 − 2.5 µg/kg [150]
Hormones Animal manure, mushroom compost, and biosolids SPE-GC/MS Not available [56]
Pesticides Cropland soils HPLC-MS/MS coupled with GC–MS/MS 0.01 − 0.30 ng/L [45]
Pesticides Vegetables GC-MSD 0.01 − 0.07 mg/kg [39]
Steroids Swine farms SPE-ESI-UHPLC-MS/MS Not available [59]
Pesticides Chinese vegetables GC–MS and UHPLC-MS/MS 0.001 − 0.005 mg/kg [35]
Organophosphorus pesticides Soil USL-SPE/DSLLME 0.012 and 0.2 ng/g [151]

DSLLME, dispersive-solidification liquid–liquid microextraction; ECD, electron capture detector; ESI, electrospray ionization; G.C., gas chromatography; GC–MSD, gas chromatography coupled with a mass selective detector; GC–MS/MS, gas chromatography triple quadrupole mass spectrometry; HPLC-UV, high-performance liquid chromatography coupled to an ultraviolet detector; HPLC, high-performance liquid chromatography; LC-MS/MS, liquid chromatography coupled with tandem quadrupole mass spectrometry; LC- ESI-QqQ, liquid chromatography coupled with triple quadrupole mass spectrometer; MALDI-HRMS, matrix-assisted laser desorption/ionization high-resolution mass spectrometry; MRM, multiple reaction monitoring; SPE, solid phase extraction; UHPLC-MS/MS, ultra-high-performance liquid chromatography tandem mass spectrometry; UHPLC-QqLIT-MS, ultra-high-performance-liquid chromatography coupled to a quadrupole-linear ion trap tandem mass spectrometer; USL-SPE, ultrasound leaching-solid phase extraction.