Figure 3.

Genomic and RT-PCR products derived from D. teissieri jgw gene in an intron-present and an intron-absent line. (A) PCR reactions were performed from gDNA (lanes 1 and 3) and cDNA (lanes 2 and 4) with primers surrounding intron 2 in the ymp-derived region after a first round of amplification with a jgw-specific primer. The size of the products in base pairs (bp) is indicated on the right. (B) Genomic sequence of the region surrounding intron 2. Coding and intronic sequences are displayed as capital and small letters, respectively. Amino acids are presented above the second position of each codon. The nucleotide sequence corresponding to the four new amino acids acquired by exon 2 is displayed inside the open box.