Table 2.
Comparison of experimental binding data with predicted binding affinity
| p50p50
|
p50p65
|
|||
|---|---|---|---|---|
| Predicted | Observed | Predicted | Observed | |
| GGAGCCCTCC | 0.043 | 0.016 | 0.020 | 0.016 |
| GGAAGATTCC | 0.070 | 0.059 | 0.066 | 0.117 |
| GGAAACCTCC | 0.102 | 0.125 | 0.074 | 0.109 |
| GGAAGATCCC | 0.414 | 0.481 | 0.379 | 0.422 |
| GGAGTTCCCC | 0.656 | 0.613 | 0.703 | 0.660 |
| GGAGAGCCCC | 0.570 | 0.594 | 0.555 | 0.613 |
| GGGATGCCCC | 0.871 | 0.863 | 0.820 | 0.789 |
| GGGAGTCCCC | 0.883 | 0.895 | 0.887 | 0.828 |
| GGGGATCCCC | 0.988 | 0.988 | 0.938 | 0.953 |
Nine consensus sequence variants were tested by EMSA with recombinant p50 and p50RHDs/p65RHDs. Predicted values are represented by rank, on a scale of 0–1, within the 256 sequences examined by the PC model. Observed values are represented by rank within the total set of sequences tested experimentally.