Table 3.
Analysis of signal and coding joining in H2AXΔ/Δ ES cells
| Cell lines | (Ampr + Camr)/Ampr | % | Relative level | Fidelity (%) |
|---|---|---|---|---|
| pJH290 (coding joining) | ||||
| Exp. I | ||||
| TC1 | 702/234,500 | 0.3 | 1.0 | |
| H2AXFlox/Δ (no. 40) | 668/188,000 | 0.4 | 1.3 | |
| H2AXFlox/Δ (no. 79) | 760/197,500 | 0.4 | 1.3 | |
| H2AXΔ/Δ (no. 45) | 456/94,000 | 0.5 | 1.7 | |
| H2AXΔ/Δ (no. 85) | 533/109,000 | 0.5 | 1.7 | |
| XRCC4−/− | 0/124,000 | <0.0008 | <0.002 | |
| Exp. II | ||||
| H2AXFlox/Δ (no. 40) | 205/45,200 | 0.5 | 1.0 | |
| H2AXΔ/Δ (no. 43) | 1,268/182,200 | 0.7 | 1.4 | |
| H2AXΔ/Δ (no. 45) | 170/32,200 | 0.6 | 1.2 | |
| XRCC4−/− | 5/85,400 | 0.006 | 0.01 | |
| pJH200 (signal joining) | ||||
| Exp. I | ||||
| TC1 | 1,168/511,000 | 0.2 | 1.0 | ND |
| H2AXFlox/Δ (no. 40) | 1,096/727,000 | 0.2 | 1.0 | ND |
| H2AXFlox/Δ (no. 79) | 1,436/550,000 | 0.3 | 1.5 | ND |
| H2AXΔ/Δ (no. 45) | 633/278,500 | 0.2 | 1.0 | 15/15 (100%) |
| H2AXΔ/Δ (no. 85) | 506/285,000 | 0.2 | 1.0 | 14/14 (100%) |
| XRCC4−/− | 5/578,000 | 0.0009 | 0.0004 | ND |
| Exp. II | ||||
| H2AXFlox/Δ (no. 40) | 210/115,000 | 0.2 | 1.0 | |
| H2AXΔ/Δ (no. 43) | 1,332/425,000 | 0.3 | 1.5 | |
| H2AXΔ/Δ (no. 45) | 184/94,733 | 0.2 | 1.0 | |
| XRCC4−/− | 7/64,533 | 0.01 | 0.05 |