Table 2.

Effect of various concentrations of substrate on activity of free and immobilized enzyme preparations

L-Tyrosine (Substrate Conc.) mg/mL	L-Tyrosine (Substrate Conc.) mM	Free L-Tyrosine Hydroxylase (U/mL)	Immobilized L-Tyrosine Hydroxylase (U/mL)	1/[S]	1/V (Free Enzyme)	1/V (Immobilized Enzyme)
0.156	8.61 × 10− 4	0.37	0.99	1161.47	2.70	1.01
0.312	1.72 × 10− 3	0.72	1.9	580.74	1.39	0.53
0.625	3.45 × 10− 3	1.4	3.75	289.90	0.71	0.27
1.25	6.90 × 10− 3	2.83	7.48	144.95	0.35	0.13
2.5	1.38 × 10− 2	5.62	14.87	72.48	0.18	0.07
5	2.76 × 10− 2	5.62	14.87	36.24	0.18	0.07
10	5.52 × 10− 2	5.62	14.87	18.12	0.18	0.07