Figure 4.
High-performance thin-layer chromatography profile of total cellular lipids extracted from L5178Y-R cells. L5178Y-R cells were cultured for 5 days in vehicle (0.05% ethanol; lane A) or 10 μM PPMP (lane B). The pelleted cells were washed and extracted with C:M (2:1, vol/vol) by sonication. Contaminating phospholipids were removed by alkaline saponification (methanolic alkali). Cell equivalents were spotted for comparative analysis. The chromatogram was developed in C:M:W (65:25:4, vol/vol/vol), and glycolipids were detected by orcinol spray. The arrow indicates the standard relative mobility (Rf) of Gg3Cer. C:M:W, chloroform/methanol/water.