Figure 2.

Tg CD25⁺4⁺ cells from T/R⁺ mice exhibit MBP Ac1–17 peptide-specific suppressive activity in vitro. CD25⁻4⁺ cells (2.5 × 10⁴) sorted from T/R⁺ mice were cultured for 3 days with 2.5 × 10⁴ antigen-presenting cells and 3 μM MBP Ac1–17 peptide (pep) (Left) or 1 μg/ml of anti-CD3 mAb (Right) in the presence or absence of 2.5 × 10⁴ 3H12⁻CD25⁺4⁺ or total CD25⁺4⁺ cells sorted from T/R⁺ cells.