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SF-1 is a nuclear protein independently of the presence of wild-type or AHC mutants DAX-1. Double-color immunofluorescence showing localization of transfected SF-1 (green) in HeLa cells, in the absence and the presence of transfected wild-type, R267P, ΔV269, and N440I DAX-1 (red). DNA was stained by the Hoechst 33342 dye (blue). (Magnification: ×600.)
