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. 2002 Jun 4;99(12):8242–8247. doi: 10.1073/pnas.112218799

Figure 4.

Figure 4

Detection of TEL-AML1 and AML1-ETO fusion transcripts in normal cord blood samples by real-time quantitative PCR (Taqman). Shown is a linear detection of TEL-AML1 (A) and AML1-ETO (B) fusion transcripts (in REH and Kasumi-1 cell lines, respectively) over at least four and five logs, respectively. This assay could detect one TEL-AML1-positive cell in a background of 10,000 negative cells and one AML1-ETO-positive cell in a background of 100,000 negative cells. Cycle threshold values and positive cord bloods indicated with B or C numbers were used to calculate appropriate cell frequency (10−3 to 10−4) from standard.