9. Affinity of NLRP3 Inhibitors from Cell-Based NanoBRET Tracer Competition Binding Experiments in Human HEK293 Cells.

	binding affinity K i  (nM) (n) [pK i ± SD]
inhibitor	MCC950 analogue as tracer	28 as tracer
MCC950	14 (5) [7.6 ± 0.3]	19 (6) [7.7 ± 0.2]
4	18 (5) [7.8 ± 0.3]	11 (6) [7.9 ± 0.1]
17	2.7 (3) [8.6 ± 0.1]	4.0 (6) [8.4 ± 0.1]
21	4.3 (5) [8.4 ± 0.2]	2.3 (5) [8.6 ± 0.1]
25	14 (5) [7.9 ± 0.3]	16 (6) [7.8 ± 0.1]

^aThe geometric mean of replicate experiments is reported.