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(A) Immunoblot showing the time-dependent effect of HG (30 mM) and simvastatin (1 μM) on CDK inhibitor p21 after 6, 12, and 24 h of treatment. (B) Immunoblots showing the time-dependent effects of HG (30 mM) and simvastatin (1 μM) on the expression of cell-cycle regulatory proteins cyclin A and D1 and CDK inhibitor p27 after 6, 12, and 24 h of exposure. The blots in A and B are representative of three separate experiments.
