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. 2002 Jun 4;99(12):8389–8393. doi: 10.1073/pnas.122206399

Figure 4.

Figure 4

cAMP-induced potentiation mimics, occludes, and is necessary for 4 Hz but not 1 Hz LTP. (a) Application of forskolin (50 μM) for 10 min (shaded region) induced LTP (n = 5). (b) Preexposure of slices to 50 μM forskolin for 10 min 1–2 h before the onset of recording occluded 4 Hz LTP induction (□, n = 6), whereas 1 Hz LTP was still inducible (●, n = 4). Whole-cell recording during the development of the forskolin effect was avoided for the same reasons as in Fig. 2d. (c) 1 Hz and 4 Hz LTP induction protocols were applied to cerebellar slices from mice lacking adenylyl cyclase types 1 and 8. One Hz LTP was induced normally (●, n = 6), whereas 4 Hz stimulation gave only a transient increase in EPSCs (□, n = 6). (d) Application of the protein kinase A inhibitor KT5720 (5 μM, shaded region) 5 min before and during LTP induction prevented 4 Hz LTP (□, n = 7) but not 1 Hz LTP (●, n = 11).