Figure 3.

Difference in molecular determinants for interdomain interactions in CNGA1/CNGB1 channels versus CNGA2 channels. (A) Cartoon showing bait proteins derived from CNGA2 (gray) and CNGB1 (red) and fish proteins derived from CNGA1 (blue) located directly beneath the corresponding region of a generic CNG subunit. The exact amino acid composition of the fusion proteins are given by the numbers within each associated name. (B) Western blot of biochemical pull-down interaction using CNGB1#677–764 as bait and CNGA2#1–138 as fish. (C) Western blot using CNGA2-derived protein as bait and the same fish proteins as in B.