Figure 4.

Requirement of N-terminal Ca²⁺/CaM binding site in CNGB1 for binding both Ca²⁺/CaM and a C-terminal domain of CNGA1. (A) Cartoon showing bait and fish fusion proteins and their position relative to a generic CNG channel subunit. (B) Gel showing results of biochemical pull-down interaction assay between bait proteins CNGB1#677–764 and CNGB1#702–764 and fluorescently labeled CaM in the presence of Ca²⁺ or EDTA. (C) Western blot showing results of pull-down assays with bait proteins CNGB1#677–764 and CNGB1#702–764 and the fish protein CNGA1#497–693.