FIG. 4.

Transcription and translation of BBA74 in mutant strains. (A) RT-PCR. Lanes 1 and 2, wild-type B31; lane 3, mutant B31; lanes 4 and 5, wild-type BL206; lane 6, mutant BL206; lanes 7 and 8, wild-type B356; lane 9, mutant B356. Reactions depicted in lanes 1, 4, and 7 lacked reverse transcriptase. Amplified products were separated by electrophoresis on a 1.5% agarose gel and stained with ethidium bromide. Lane M, DNA molecular size markers. (B) Immunoblotting. Total cell lysate (3.5 μg) was separated by 12.5% SDS-PAGE and transferred to polyvinylidene difluoride membranes, and blots were developed with either OspA/B-specific or BBA74-specific antibodies. P, wild type; M, mutant.