TABLE 1.
Assessment of histopathological features after infection with different strains of M. ulcerans
Histopathological parameter | M. ulcerans strain | No. of positive cases/total studied cases on postinfection day:
|
|||||
---|---|---|---|---|---|---|---|
2 | 7 | 15 | 24 | 43 | 60 | ||
Acute inflammation (predominant neutrophilic infiltrate) | 98-912 | 5/5 | 2/6 | 6/6 | 6/6 | —a | — |
97-1116 | 3/3 | 4/6 | 5/6 | 6/6 | 2/2 | — | |
5114 | 3/3 | 2/6 | 0/6 | 0/5 | NDb | 0/6 | |
Chronic inflammation (predominant macrophagic-lymphocytic infiltrate) | 98-912 | 0/5 | 4/6 | 0/6 | 0/6 | — | — |
97-1116 | 0/3 | 1/6 | 1/6 | 0/6 | 0/2 | — | |
5114 | 0/3 | 4/6 | 6/6 | 5/5 | ND | 6/6 | |
Presence of granuloma-like structures | 98-912 | 0/5 | 0/6 | 0/6 | 0/6 | — | — |
97-1116 | 0/3 | 0/6 | 0/6 | 0/6 | 0/2 | — | |
5114 | 0/3 | 0/6 | 6/6 | 5/5 | ND | 6/6 | |
Leukocyte destruction (apoptosis/necrosis) | 98-912 | 5/5 | 6/6 | 6/6 | 6/6 | — | — |
97-1116 | 3/3 | 6/6 | 6/6 | 6/6 | 2/2 | — | |
5114 | 0/3 | 0/6 | 0/6 | 0/5 | ND | 0/6 | |
Subcutaneous tissue necrosis | 98-912 | 0/5 | 0/6 | 3/6 | 6/6 | — | — |
97-1116 | 0/3 | 0/6 | 3/6 | 6/6 | 2/2 | — | |
5114 | 0/3 | 0/6 | 0/6 | 0/5 | ND | 0/6 |
—, animals were sacrificed after emergence of ulceration.
ND, not done.