FIG. 3.

Proteins eluted from the surface S. epidermidis 0-47 (grown in TSB or 70% rabbit serum) with increasing concentrations of NaCl or 4.0 M urea. Bacteria were washed three times with TBS and then sequentially with 0.5 M and 1.0 M NaCl and 4.0 M urea. Protein concentrations were determined for each of the samples, and 0.75 μg was run on a 4 to 20% gradient gel. No protein was detected by protein assay in the samples eluted from the surface of TSB-grown bacteria (lanes 2 to 5). As a result, 25 μl of these samples was run on the gel. Lane 1, rabbit serum; lanes 2 and 5, to 0.5 M NaCl, lanes 3 and 6, to 1.0 M NaCl; lane 4 and 7, to 4.0 M urea. Asterisks indicate enriched proteins eluted from the surface of S. epidermidis grown in the presence of serum.