. 2005 Oct;73(10):6591–6600. doi: 10.1128/IAI.73.10.6591-6600.2005

TABLE 1.

List of spots identified^a

Spot no.	Predicted function^b	ATCC 12228 equivalent ORF (GenBank)	Method of detection^d	Log CFU Reduction^e
Spot no.	Predicted function^b	ATCC 12228 equivalent ORF (GenBank)	Method of detection^d	Spleen	Blood
1, 2	Dihydrolipoamide dehydrogenase	gi\|27467712	I,S	0	0
3	Dihydrolipoamide dehydrogenase	gi\|27467712	I,S
	Glutamate-1-semialdehyde 2,1-aminomutase	gi\|27468260	I,S	0	0
4	Enolase	gi\|27467479	I,S	1.5	0.7
	Elongation factor TU	gi\|27467230	I,S	0	0
5-7, 9	Enolase	gi\|27467479	I,S
8	Phosphogluconate dehydrogenase	gi\|27468110	I	0	0
10	Enolase	gi\|27467479	I,S
	Elongation factor TU	gi\|27467230	I,S
	Na⁺/H⁺ antiporter	gi\|27468791	I,S	0.9	0
11	Alanine dehydrogenase	gi\|27468302	I,S	0	1
12	GAPDH	gi\|27467475	I,S	0	0
13	GAPDH	gi\|27467475	I,S
	Elongation factor TS	gi\|27467851	I,S	0	0
14	No match		I,S
15-16	GAPDH	gi\|27467475	I,S
17	Acetyl-CoA C-acetyltransferase	gi\|27469302	I	1.2	0.9
18	Cystathionine gamma-synthase	gi\|27469241	I	0	0
19	Ferrichrome binding lipoprotein	gi\|27468686	I,S	0	0
	Oligopeptide permease	gi\|27467598	I,S	NT	NT
20-23, 25	Ferrichrome binding lipoprotein	gi\|27468686	I,S
24	Fructose-bisphosphate aldolase	gi\|27469074	I,S	0	0
26	Fructose-bisphosphate aldolase	gi\|27469074	I,S
	Hypothetical protein	gi\|27469180	I,S	0	0
	Hypothetical protein	gi\|27468551	I,S	0.5	0
27, 30-32	Lipoprotein (SitC)	gi\|27467323	I,S	0.8	1
28, 33	Amino acid-binding lipoprotein	gi\|27468911	I,S	2	0
29	Putative hexulose-6-phosphate synthase	gi\|27467259	I,S	0.5	0
34	Lipoate ligase	gi\|27467278	I,S	1.1	0
35	No match		I
36	No match		I
37	No match		I,S
38	IsaA	gi\|27469044	I,S	0	0
39	Embp	gi\|27468046	I,S	NT	NT
40	Cysteine synthase	gi\|27469188	S	1	0.8
	Fructose-bisphosphate aldolase homologue	gi\|27468641	S	1	0
	Thioredoxine reductase	gi\|27467465	S	0	0
41	Cysteine synthase	gi\|27469188	S
42	Putative transaldolase	gi\|27468367	I	0	0
43	Embp	gi\|27468046	S	NT	NT
	Transketolase	gi\|27467943	S	0	0
	Hypothetical protein	gi\|27467889	S	0	0
44	Glutamyl-tRNAGln amidotransferase subunit	gi\|27468502	S	0	0

List of spots detected on 2D blots by reactivity with immune sera or binding to serum components. Corresponding ORFs in Incyte's PathoSeq S. epidermidis 0-47 database were identified by analysis of mass spectrometry data using MSFIT (Protein Prospector) and MASCOT (Matrix Science) database search engines.

The predicted function of the proteins was determined by comparison with complete genome homologues from ATCC 12228 (44).

The GenBank accession numbers from ATCC 12228 are listed.

Method by which the spot was detected following transfer to nitrocellulose, reactive immune sera from infected rabbits (I) or binding serum components (S).

Mice (five animals/group) vaccinated with the recombinant proteins were challenged with 3 × 10⁸ CFU of S. epidermidis 0-47 and the bacteria in the spleen and blood were enumerated 24 h postinfection. The data are reported as the log reduction in bacteria compared to data for mice vaccinated with adjuvant control alone, where 5.5 to 6.4 log₁₀ CFU were recovered from spleen and 2.5 to 3.5 log₁₀ CFU were recovered per ml of blood. NT, not tested.