TABLE 2.
Summary of challenge dataa
| Antigen | Log10 CFU reductiona
|
|
|---|---|---|
| Spleen | Bloodc | |
| Na+/H+ antiporter | 1.2* | NT |
| Alanine dehydrogenase | 0.8 | 1.2** |
| Enolase | 0.9 | NT |
| Fructose bisphosphate aldolase homologue | 0.5 | NT |
| Acetyl-CoA C-acetyltransferase | 1.4* | NT |
| Hypothetical | 0.3 | NT |
| Lipoate ligase | 1.6** | NT |
| Putative hexulose-6-phosphate synthase | 0 | NT |
| SitC | 0.5 | NT |
| Amino acid binding lipoprotein | 0.8 | NT |
| Cysteine synthase | 1.2* | 1.2** |
Groups of 10 female (4-week-old) BALB/c mice were vaccinated by subcutaneous injection with saline or 10 μg of antigen with 20 μg of QS21 as adjuvant: Two weeks following the last vaccination, the mice were challenged with ≈5 × 108 CFU S. epidermidis 0-47 by intraperitoneal injection. Twenty-four hours after challenge, the bacteria in the blood and spleen were enumerated.
Reduction in CFU is determined as compared to a control of QS21 in saline with recoveries of 5.5 to 6.4 log10 CFU/spleen and 2.5 to 3.5 log10 CFU/ml of blood. Data analyzed by Student's t test with resulting P values of 0.05 (*) or 0.01 (**).
The numbers of CFU in the bloodstream were not tested (NT) for most of the antigens based upon initial results (Table 1), which indicated that attaining statistical significance would be unlikely.