FIG.1.

Gel filtration elution profiles of PNAG-DT (A) and dPNAG-DT conjugate vaccines (B) on a Superose 6 prep-grade column. The presence of polysaccharide (open circles) in fractions was monitored by the hexosamine assay (OD₆₅₀) and the presence of protein (closed circles) in the fractions was monitored by the Bradford assay (OD₅₉₅). Double-headed arrows indicate the fractions containing clearly conjugated protein and polysaccharide, as well as where the unconjugated polysaccharide (PNAG or dPNAG) and protein (DT) eluted.