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Mean titers of IgG antibodies in sera pooled from 10 mice immunized three times at weekly intervals with the indicated dose of PNAG-DT (A) or dPNAG (B) conjugate vaccines. Sera were collected weekly for 4 weeks starting 1 week after the last dose of conjugate vaccine and were tested by ELISA with the homologous immunizing antigen, either PNAG (A) or dPNAG (B) as a coating antigen. Bars represent means and error bars indicate standard deviations. All preimmune titers were <25.
