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Diagram of the L. monocytogenes PrfA regulon and the mutants constructed in the present study. The star indicates a nonfunctional H86A mutation in L. monocytogenes PI-PLC (1), the black arrows indicate the replacement of L. monocytogenes PI-PLC with B. anthracis PI-PLC, and the gray arrows indicate the replacement of LLO with ALO. BaPI-PLC, B. anthracis PI-PLC.
