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Immunological characterization of rabbit sera against rHCR/A1 and rHCR/EB. (A) ELISA of rabbit antisera to rHCR/A1 (left panel) or rHCR/EB (right panel), using 100 ng rHCR/A1, 100 ng rHCR/A2, 100 ng rHCR/ΔA, 100 ng rHCR/EB, and 100 ng rHCR/ΔE as capture antigens. (B) Antigens (500 ng) were separated by SDS-PAGE under reducing conditions and visualized by Western blotting with anti-rHCR/A1 sera (left panel) or anti-rHCR/EB (right panel).
