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Purification profile for E. coli-expressed rHCR/Aa
| rHCR/A | Total rHCR/A (mg/liter culture)b | Total protein (mg/liter culture)c | Purification factorc | Yield (%) |
|---|---|---|---|---|
| Extraction | 17 | 525 | 1 | 100 |
| Ni-NTA | 15 | 18 | 14 | 88 |
| Gel filtration | 13 | 15 | 15 | 76 |
| Ion exchange | 12 | 12 | 44 | 71 |
Data are representative of two independent determinations.
Estimated from band intensity on SDS-PAGE gels.
Based on total protein content.
