TABLE 2.
Protection from BoNT intoxication by rHCRa
| Immunization (2.5 μg antigen) and challenge | Toxin
|
||
|---|---|---|---|
| BoNT/A1 | BoNT/A2 | BoNT/EA | |
| HCR/A1 | |||
| LD50 | |||
| 10 | +,+ | +,+ | −,− |
| 100 | +,+,+,+ | +,+,+,+ | −,− |
| 1,000 | +,+ | +,+ | −,− |
| 100,000 | +,+,+,+ | +,+,+,+ | −,− |
| HCR/E | |||
| 10 | −,− | −,− | +,+ |
| 100 | −,− | −,− | +,+,+,+ |
| 1,000 | −,− | −,− | +,+ |
| 100,000 | −,− | −,− | +,+,+,+ |
a
Mice were immunized with the indicated serotype of rHCR and then challenged by the indicated amount and serotype of BoNT. Individual mice were inspected for 96 h and scored for survival (+) or death (−).