TABLE 3.
Gene downregulated by TRAPa
| Affymetrix Probe set no. | N315 ORFb | N315 genec | N315 description | Repeat 1 | Repeat 2 | Repeat 3 | H66A |
|---|---|---|---|---|---|---|---|
| c7698s | SA0519 | sdrC | Ser-Asp rich fibrinogen-binding bone sialoprotein-binding protein | 1 | − | 1.4 | − |
| c8852s | SA0520 | sdrD | Ser-Asp rich fibrinogen-binding bone sialoprotein-binding protein | 1.4 | 2.9 | 4.1 | 3.3 |
| c1007s | SA2423 | clfB | Fibrinogen-binding protein precursor | − | 1.7 | − | − |
| c1082S | SA0107 | spa | Immunoglobulin G-binding protein A | − | 2 | 4.4 | 2.2 |
| c4116s | SA1836 | groEL | 60-kDa chaperonin (GroEL protein) | 2.4 | 1.4 | − | − |
| c4124s | SA1837 | groES | 10-kDa heat shock protein (GroES protein) | 2.5 | 1.5 | − | − |
| c2703s | SA1408 | dnaJ | Immunoreactive heat shock protein DnaJ | 1.9 | 1.6 | − | − |
| c8390s | SA0728 | pgk | Phosphoglycerate kinase 2 | 1 | 1.4 | − | − |
| c5588s | SA2189 | nirR | HP, similar to NirR | 1.3 | 3.1 | − | 2.1 |
| c3173s | SA1537 | HP, similar to thiamine biosynthesis protein ThiI | 1.5 | 1.5 | − | − | |
| c5246s | Glutamate permease | 1.2 | 4.2 | 1.3 | 3.7 | ||
| c9058s | SA0904 | HP, probable ATL autolysin transcription regulator | − | 1.4 | 1.1 | 2.5 | |
| c6262s | SA1676 | HP, similar to regulatory protein (PfoS/R) | − | 1.2 | 1.1 | − | |
| c9380s | SA2303 | HP, similar to membrane-spanning protein | − | 3.8 | 2.5 | 3.1 | |
| c8053s | SA0097 | HP, similar to AraC/XylS family transcriptional regulator | 1.4 | 1.6 | − | 1.6 |
a
Log fold change of TRAP− mutant compared with parent 8325-4 TRAP+ (repeats 1, 2, and 3) and log fold change of mutant His66 (H66A) compared with parent TRAP+. A minus sign indicates no difference between control and mutant.
b
Designed S. aureus GeneChip ORF number.
c
Based on the published sequence of strain N315 (24).